Combining Ultracentrifugation and Peptide Termini Group-specific Immunoprecipitation for Multiplex Plasma Protein Analysis
نویسندگان
چکیده
منابع مشابه
High-throughput analysis of protein/peptide complexes by immunoprecipitation and automated LC-MS/MS.
BACKGROUND The identification of interacting proteins within protein complexes is key to understanding the transduction and regulation of cell signaling pathways, and is also a useful tool for identifying novel disease markers. Immunoprecipitation of protein complexes followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been used to identify targets that bind to a protein o...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2012
ISSN: 1535-9476
DOI: 10.1074/mcp.o111.015438